Protein_Domain

Part:BBa_K2809010

Designed by: TANG YU-CHENG   Group: iGEM18_CCU_Taiwan   (2018-07-11)


mature Lac 1(laccase)

This is the codon-optimized version of laccase derived from Pinus taeda(Loblolly pine). Laccase is involved in monolignol dehydrogenation, using oxygen to oxidize their metal centers to enable catalytical phenol oxidation.It is able to produce dimers and oligomers of coniferyl alcohol, which is involved in the early stage of lignification.

Note : The signal peptide have removed, so the sequence do NOT contain start codon. Furthermore, it includes HA-tag for Western Blot protein detection and 6*His-tag for protein purification before stop codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None